Anisomycin

SKU: orb1300493

Description

Anisomycin is a Streptomyces-derived antibiotic that inhibits protein synthesis, thereby inducing cellular stress and activating p38 MAPK and JNK pathways. This small molecule is widely used in research to study stress signaling, apoptosis, and cellular responses in both in vitro and in vivo models.

Research Area

Cell Biology

Key Properties

• CAS Number: 22862-76-6
• MW: 265.3
• Purity: >98%
• Formula: C₁₄H₁₉NO₄
• SMILES: COc1ccc(CC2NCC(O)C2OC(C)=O)cc1
• Target: Kinase
• Solubility: Soluble in DMSO (up to 25 mg/ml).

Bioactivity

• Target IC50:
  U-87MG cells growth:0.192 ± 0.018 μM|peripheral lymphocytes:25.44 ng/mL|U251 cells growth:0.233 ± 0.021 μM
• In Vivo:
  Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.
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• In Vitro:
  Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells. In U251 and U87 cells, anisomycin (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. Anisomycin (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells. Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.
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• Cell Research:
  For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 μL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Antibiotic, Anisomycin, Bacterial, DNASynthesis, DNA Synthesis, DNA/RNA Synthesis, Flagecidin, Inhibitor, NSC 76712, inhibit, JNK, NSC76712, NSC-76712, RNA Synthesis, RNASynthesis, Wuningmeisu C

Compound Identifiers

PubChem CID

2199