Ampicillin

SKU: orb1310267

Description

Ampicillin

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 69-53-4
• MW: 349.4
• Purity: 99.59%
• Formula: C₁₆H₁₉N₃O₄S
• SMILES: [H][C@@]1(NC(=O)[C@H](N)c2ccccc2)C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@]12[H]
• Target: Antibiotic,Antibacterial
• Solubility: 0.1 M NaOH:25 mg/mL (71.55 mM);DMSO:240 mg/mL (686.89 mM)

Bioactivity

• Target IC50:
  transporter:9.9 mM (Ki)|carnitine/carnitine antiport:7.6 mM|enzyme transpeptidase:1.8 µg/mL|HEK293 cells:> 100 μM (CC50)
• In Vivo:
  METHODS: To test the neuroprotective mechanism in a mouse model of transient total forebrain ischemia, Ampicillin (200 mg/kg) was injected intraperitoneally into C57BL/6 mice once a day for five days, and forebrain ischemia was induced 24 h after the last injection. RESULTS: Pretreatment with Ampicillin significantly attenuated neuronal damage in the CA1 subregion of the hippocampus, and Ampicillin may provide neuroprotection against cerebral ischemia/reperfusion injury by inducing GLT-1 protein and inhibiting MMP activity in the hippocampus of mice. METHODS: To study the in vivo activity, Ampicillin (10-100 mg/kg) was administered by gavage to C57BL/6 mice once daily for two days. RESULTS: Exposure of mice to Ampicillin induced anxiety and colitis, and increased Aspergillus flora in the gut microbiota.
• In Vitro:
  METHODS: E. coli was treated with Ampicillin (0.1-10 μG/mL) for 6 h. Growth was measured using a Fisher Electrophotometer. RESULTS: No differences in E. coli growth were observed at concentrations of 0, 0.1, and 1.0 μG. 2.5, 5, and 10 μG concentrations of Ampicillin inhibited E. coli growth. METHODS: Pharyngeal cancer cells Detroit-562 were treated with Ampicillin (10-100 μM) for 72 h. Cell viability was measured by MTT. RESULTS: The Ampicillin-treated groups at concentrations of 10, 25 and 50 μM showed higher cell viability than the control cells, with values of 112%, 107% and 106%, respectively. At concentrations of 75 and 100 μM, there was a slight but not significant decrease in cell viability with values of approximately 95% and 94%, respectively.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bacterial, Amcill, Ampicillin acid, Aminobenzylpenicillin, Antibiotic, D-a-Aminobenzylpenicillin, D-alpha-Aminobenzylpenicillin, D-(-)-α-Aminobenzylpenicillin, D-α-Aminobenzylpenicillin, PBPs

Compound Identifiers

PubChem CID

6249