Aminoglutethimide

SKU: orb1309763

Description

Aminoglutethimide (BA-16038) is a steroidal aromatase inhibitor used in research related to hormone-dependent cancers. It is applied in both in vitro and in vivo studies to investigate estrogen biosynthesis inhibition, particularly in models of advanced breast cancer.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 125-84-8
• MW: 232.28
• Purity: 99.98% (May vary between batches)
• Formula: C₁₃H₁₆N₂O₂
• SMILES: CCC1(CCC(=O)NC1=O)c1ccc(N)cc1
• Target: Aromatase,Cytochromes P450
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.61 mM);DMSO:55 mg/mL (236.78 mM);Ethanol:7 mg/mL (30.14 mM)

Bioactivity

• Target IC50:
  Aromatase:10 μM
• In Vivo:
  Aminoglutethimide accelerates its own metabolism from a basal value of 2.6±0.3 (S.E.) liters/24 hours to 5.3±1.4 liters/24 hours after 1 to 2 weeks of Aminoglutethimide administration, and markedly accelerates the metabolism of the synthetic glucocorticoid and dexamethasone, from basal values of 145±26.6 liters/24 hours to 568±127 liters/24 hours (p < 0.02) after 2 weeks of Aminoglutethimide administration. Aminoglutethimide (150 mg/kg) abolishes the induction of ornithine decarboxylase (ODC) and almost depletes the gonads and plasma of progesterone or testosterone elicited by human chorionic gonadotropin (hCG) in the ovary of adult female mice and the testis of immature male mice, which is related to an inhibition of cAMP-dependent protein kinase (IC50 287 μM) rather than blockade of the steroidogenic pathway.
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• Cell Research:
  The NCI-h295 tumor cell line is maintained in RPMI 1640 medium supplemented with transferrin (0.1 mg/mL), insulin (5 μg/mL), selenium (5.2 μg/mL) and 2% FCS. The cells are incubated for 48 hours with Aminoglutethimide (3, 30, 300 μM). Then cells are examined by trypan blue staining for cell viability, counted with a coulter counter. For the assessment of ACTH-R mRNA, cells are harvested, and total RNA is extracted, electrophoresed, blotted and hybridized with a human ACTH-R cDNA probe.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aromatase, BA 16038, BA16038, BA-16038, Aminoglutethimide, DL-Aminoglutethimide, Inhibitor, NSC 330915, inhibit, NSC330915, NSC-330915

Compound Identifiers

PubChem CID

2145