Aminoallyl-dUTP-XX-Texas Red

• Catalog Number: orb1733862
• Category: Tools
• Description: Aminoallyl-dUTP-XX-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-Texas Red/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-Texas Red/80-70% dTTP (PCR), 50% Aminoallyl-dUTP-XX-Texas Red/50% dTTP (Nick Translation).
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1451.39 g/mol (free acid); Detected MW: 1450.41 g/mol (free acid)
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCCNS(=O)(=O)c1cc(c(cc1)C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-].P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCCNOS(=O)(=O)c1cc(ccc1C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-]
• Formula: C₆₁H₈₁N₈O₂₃P₃S₂
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 588 nm, λem 609 nm, ε 80.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.