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Aminoallyl-dUTP - Solution
Catalog Number: orb1733876
| Catalog Number | orb1733876 |
|---|---|
| Category | Small Molecules |
| Description | Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging. |
| Form/Appearance | solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
| Concentration | 100 mM-110 mM |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid) |
| Application notes | Incorporation into DNA/cDNA by PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| CAS Number | 116840-18-7 |
| Formula | C12H20N3O14P3 |
| SMILES | NC/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O |
| Storage | store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Aminoallyl-dUTP - Solution [orb1733872]
≥ 95% (HPLC)
116840-18-7
Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid)
C12H20N3O14P3
100 μlAminoallyl-dUTP - Solution [orb1733874]
≥ 95% (HPLC)
116840-18-7
Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid)
C12H20N3O14P3
10 μl
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
