Amantadine hydrochloride

SKU: orb1224297

Description

Amantadine Hydrochloride is an antiviral and an antiparkinsonian drug.(In Vitro):Amantadine hydrochloride (0-500 μM, 26 h) inhibits SARS-CoV-2 replication, with IC50 concentrations between 83 and 119 μM.Amantadine hydrochloride (0-100 μg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC 7721 cells.Amantadine hydrochloride (0-75 μg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis.Amantadine hydrochloride (0-75 μg/mL, 48 h) reduces the levels of the cell cycle related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels.(In Vivo):Amantadine hydrochloride (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 665-66-7
• MW: 187.71
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₈ClN
• SMILES: Cl.NC12CC3CC(CC(C3)C1)C2
• Target: Dopamine Receptor
• Solubility: Ethanol: 38 mg/mL (202.45 mM); Water: 38 mg/mL (202.45 mM); DMSO: 38 mg/mL (202.45 mM)

Bioactivity

• In Vivo:
  Animal model: Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group). Dosage: 25 mg/kg. Administration: IP, once daily for 3 days (the first dose at 15 min before surgery). Result: Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.
• In Vitro:
  Cell Viability Assay Cell line: Vero E6 cells. Concentration: 500 μM, 100 μM, 20 μM, 4 μM, and 8 nM. Incubation time: 26 h. Result: Caused a concentration-dependent reduction (IC50 = 83 μM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 μM. Caused a concentration-dependent reduction (IC50 = 119 μM) of viral nucleic acids in the cytosol 26 h after infection. Cell Proliferation Assay Cell line: Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells). Concentration: 0, 1, 2, 5, 10, 25, 50 and 100 μg/mL. Incubation time: 24, 48 and 72 h. Result: Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells. Cell Cycle Analysis Cell line: HepG2 and SMMC-7721 cells. Concentration: 0, 10, 25, 50 and 75 μg/mL. Incubation time: 48 h. Result: Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase. Apoptosis Analysis Cell line: HepG2 and SMMC-7721 cells. Concentration: 0, 10, 25, 50 and 75 μg/mL. Incubation time: 48 h. Result: Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner. Western blot analysis. Cell line: HepG2 and SMMC-7721 cells. Concentration: 0, 10, 25, 50 and 75 μg/mL. Incubation time: 48 h. Result: Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells. RT-PCR Cell line: HepG2 and SMMC-7721 cells. Concentration: 0, 10, 25, 50 and 75 μg/mL. Incubation time: 48 h. Result: Revealed an increase in Bax and decrease in Bcl-2 genes.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Adamantylamine | Aminoadamantane | NSC 83653

Quick Database Links

Gene Symbol

Dopamine Receptor