Albiflorin

SKU: orb1222293

Description

Albiflorin has anticonvulsant effect for pain relief. Albiflorin and paeoniflorin has neuroprotective action against Glu toxicity. Albiflorin and paeoniflorin significantly ameliorated Glu-induced reduction of cell viability, nuclear and mitochondrial apoptotic alteration, reactive oxygen species accumulation and B-cell lymphoma 2 (Bcl-2)/Bax ratio. Albiflorin can serve as a new chemical marker for the quality control of Paeoniae Radix and the Chinese Pharmacopoeia can be updated, via has similar anti-inflammatory effects to paeoniflorin.

Key Properties

• CAS Number: 39011-90-0
• MW: 480.46
• Purity: >98% (HPLC)
• Formula: C₂₃H₂₈O₁₁
• SMILES: CC12CC(C3CC1(C3(C(=O)O2)COC(=O)C4=CC=CC=C4)OC5C(C(C(C(O5)CO)O)O)O)O
• Target: Others
• Solubility: DMSO : ≥ 5.1 mg/mL; 10.61 mM

Bioactivity

• In Vivo:
  Albiflorin (50 mg/kg; intraperitoneal injection; once a day; for 15 days; Wistar rats) treatment significantly increases the paw withdrawal threshold (PWT) on the 11th and 15th day after surgery in rats. Albiflorin could inhibit the activation of p38 MAPK pathway in spinal microglia and subsequent upregulated IL-1β and TNF-α. Albiflorin displays remarkable effects on inhibiting the activation of astrocytes, suppressing the overelevated expression of phosphorylation of c-JNK (p-JNK) in astrocytes, and decreasing the content of chemokine CXCL1 in the spinal cord. Animal model: Wistar rats (7-week-old; 200-220 g) bearing chronic constriction injury (CCI). Dosage: 50 mg/kg. Administration: Intraperitoneal injection; once a day; for 15 days. Result: Significantly increased the paw withdrawal threshold (PWT) on the 11th and 15th day after surgery.
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• In Vitro:
  Albiflorin (50-200 μM; pretreated for 3 hours; PC12 cells) treatment significantly ameliorates glutamate (Glu)-induced reduction of cell viability. Albiflorin (100 μM; pretreated for 3 hours; PC12 cells) treatment significantly ameliorates Glu-induced reduction of nuclear and mitochondrial apoptotic alteration, reactive oxygen species accumulation, and B-cell lymphoma 2 (Bcl-2)/Bax ratio. Albiflorin (100 μM; pretreated for 3 hours; PC12 cells) treatment enhances phosphorylation of AKT and its downstream element GSK-3β. Cell Viability Assay Cell line: PC12 cells. Concentration: 50 μM, 100 μM, 200 μM. Incubation time: Pretreated for 3 hours. Result: Significantly ameliorated Glu-induced reduction of cell viability. Apoptosis Analysis Cell line: PC12 cells. Concentration: 100 μM. Incubation time: Pretreated for 3 hours. Result: Significantly ameliorated Glu-induced reduction of nuclear and mitochondrial apoptotic alteration. Western blot analysis. Cell line: PC12 cells. Concentration: 100 μM. Incubation time: Pretreated for 3 hours. Result: Enhanced phosphorylation of AKT and its downstream element GSK-3β.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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