Albendazole

SKU: orb1224704

Description

Albendazole is a member of the benzimidazole compounds used as a drug indicated for the treatment of a variety of worm infestations.(In Vitro):Albendazole (100, 500, 1000 nM; 1, 3, or 5 days) inhibits cell proliferation in a dose-dependent manner.Albendazole (100, 250, 500, 1000 nM; 3 days) arrests SKHEP-1 cells at both G0–G1 (250, 500 nM) and G2-M (1000 nM) phases of the cycle.Albendazole (5 μM; 24, 36 h) mainly induces early apoptosis in HCT-15 cells and late apoptosis in HCT-1 16, HT29, SW480 cells accompanied with cleavage of PARP and caspase-3 in a time-dependent manner.Albendazole (5 μM; 24, 36 h) induces autophagy via increasing autophagy-related protein (such as LC3, Atg7, p-beclin-1, and beclin-1) expression level in HCT-15, HCT-1 16, HT29, and SW480 cells.Albendazole (500 nM, 24 h) inhibits hypoxia-induced HIF-1α expression and VEGF expression in A549 cells.(In Vivo):Albendazole (10 mg/kg; i.g.; once a day for 30 days) reduces Echinococcus granulosus cyst weights in mice.Albendazole (300 mg/kg; p.o.; per day in two divided dose for 20 days) profoundly suppresses tumor growth in vivo.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 54965-21-8
• MW: 265.33
• Purity: >98% (HPLC)
• Formula: C₁₂H₁₅N₃O₂S
• SMILES: CCCSC1=CC2=C(C=C1)N=C(N2)NC(=O)OC
• Target: Microtubule/Tubulin
• Solubility: DMSO: 17mg/ml (64.07 mM); Water: <1mg/ml; Ethanol: <1mg/ml

Bioactivity

• In Vivo:
  Albendazole (10 mg/kg; i. g. ; once a day for 30 days) reduces Echinococcus granulosus cyst weights in mice. Albendazole (300 mg/kg; p.o. ; per day in two divided dose for 20 days) profoundly suppresses tumor growth In vivo. Animal model: Female BALB/c mice (10-week-age; Echinococcus granulosus infection model). Dosage: 10 mg/kg. Administration: Oral gavage; once a day for 30 days. Result: Reduced Echinococcus granulosus cyst weights. Animal model: Male BALB/c Nu/Nu mice (6 to 10-week-old; inoculated subcutaneously with SKHEP-1). Dosage: 50, 150, 300 mg/kg. Administration: Oral administration; per day in two divided dose for 20 days. Result: Profoundly suppressed tumor growth In vivo.
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• In Vitro:
  Albendazole (100, 500, 1000 nM; 1, 3, or 5 days) inhibits cell proliferation in a dose-dependent manner. Albendazole (100, 250, 500, 1000 nM; 3 days) arrests SKHEP-1 cells at both G0-G1 (250, 500 nM) and G2-M (1000 nM) phases of the cycle. Albendazole (5 μM; 24, 36 h) mainly induces early apoptosis in HCT-15 cells and late apoptosis in HCT-1 16, HT29, SW480 cells accompanied with cleavage of PARP and caspase-3 in a time-dependent manner. Albendazole (5 μM; 24, 36 h) induces autophagy via increasing autophagy-related protein (such as LC3, Atg7, p-beclin-1, and beclin-1) expression level in HCT-15, HCT-1 16, HT29, and SW480 cells. Albendazole (500 nM, 24 h) inhibits hypoxia-induced HIF-1α expression and VEGF expression in A549 cells. Cell Proliferation Assay. Cell line: SKHEP-1 cells. Concentration: 100, 500, 1000 nM. Incubation time: 1, 3, or 5 days. Result: Inhibited cell proliferation in a dose-dependent manner. Cell Cycle Analysis Cell line: SKHEP-1 HCC cells. Concentration: 100, 250, 500, 1000 nM. Incubation time: 3 days. Result: Showed dose-dependent effect on the cell cycle kinetics. Apoptosis Analysis. Cell line: HCT-15, HCT-1 16, HT29, SW480 cells. Concentration: 5 μM. Incubation time: 24, 36 h. Result: Promoted apoptosis in colon cancer cells. Cell Autophagy Assay Cell line: HCT-15, HCT-1 16, HT29, SW480 cells. Concentration: 5 μM. Incubation time: 24, 36 h. Result: Induced autophagy in colon cancer cells. Western blot analysis. Cell line: HCT-15, HCT-1 16, HT29, SW480 cells. Concentration: 5 μM. Incubation time: 12, 24, 36 h. Result: Induced apoptosis-related protein (PARP, caspase-3) and autophagy-related protein (such as LC3, Atg7, p-beclin-1, and beclin-1) expression level in a time-dependent manner. Increased phosphorylation of different MAPKs (AMPK, ERK, JNK, p38) and ULK1 protein in a time dependent manner, and up-regulated the activation of different MAPKs. Caused the activation of both MAPK and AMPK pathways. Western blot analysis. Cell line: A549 cells. Concentration: 500 nM. Incubation time: 24 h. Result: Inhibited hypoxia-induced HIF-1α expression and VEGF expression in A549 cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 220008 | SKF 62979

Quick Database Links

Gene Symbol

Microtubule/Tubulin