Alantolactone

SKU: orb1304877

Description

Alantolactone

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 546-43-0
• MW: 232.32
• Purity: 99.91%
• Formula: C₁₅H₂₀O₂
• SMILES: [H][C@@]12C[C@@]3(C)CCC[C@H](C)C3=C[C@]1([H])C(=C)C(=O)O2
• Target: STAT,TGF-beta/Smad,Apoptosis
• Solubility: DMSO:250 mg/mL (1076.1 mM);Ethanol:46 mg/mL (198 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.61 mM)

Bioactivity

• In Vivo:
  METHODS: To confir the antitumor effect of Alanolactone in vivo a xenograft experiment was performed on MDA-MB-231 cells the cells were injected into athymic nude mice and treated with alanolactone (2.5 mg/kg, intraperitoneal injection, 14 days). Result the average tumor volume of mice treated with alanolactone was approximately 2.17 times lower than that of control mice. Alantolactone administration did not affec the ove All body weight and had no obvious toxicity to mice.
• In Vitro:
  METHODS the inhibitory effects of Alanolactone (5, 10, 1 μM) o the growth of MDA-MB-231, MCF-10A and MCF-7 cells were compared. Results Alanolactone significantly inhibited cell proliferation of MDA-MB-231 cells compared with MCF-10A and MCF-7 cells. METHODS: To investigat the effect of Alantolactone (5, 10, 15 μM) o the expression levels of STAT3-related proteins in MDA-MB-231 cells, Western blot analysis of p-STAT3, STAT3, p-JAK1, JAK1, p-JAK2, JAK2, p-Src, Src, p-ERK, and ERK was performed on MDA-MB-231 cell lysates. Results Alantolactone selectively inhibited STAT3 phosphorylation, thereby suppressin the secondary signaling events of STAT3 Inhibition and inhibiting MDA-MB-231 cell proliferation. METHODS: U87, U373, and LN229 glioma cell lines were treated with alantolactone (10-60 μM) for 12 h, and cell viability was measured by MTT assay. Results Alantolactone treatment increase the growth Inhibition of U87, U373, and LN229 cells in a dose-dependent manner, with IC50 Values ​​of alantolactone for U87, U373, and LN229 cells being 33, 35, and 36 μM, respectively
• Cell Research:
  Cell viability was evaluated using MTT assay the cells were seeded into 96-well plates and maintained at 37℃or 24 h the cells were treated with alantolactone for 24 h the MTT solution (0.5 mg/mL) was added to Each well, an the cells were incubated for a Other 3 h the MTT formazan crystals were dissolved in DMSO the results were obtained by measurin the absorbance at a wavelength of 540 nM using a microplate reader.(Only for Reference)

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Alant camphor, Alantolactone, (+)-Alantolactone, Apoptosis, inhibit, Inula camphor, Eupatal, helenin, Helenine, Inhibitor, Transforming growth factor beta, TGFb, TGF-β/Smad, TGFβ, TGF-beta/Smad, TGFbeta/Smad, TGFbeta, TGF-beta, TGF-b/Smad, Smad, STAT

Compound Identifiers

PubChem CID

72724