AKT1, AKT2, AKT3 Antibody

SKU: orb344704

Description

AKT antibody

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1,000 - 1:5,000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  AKT Antibody is tested in western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Expect bands at ~56 kDa in size corresponding to AKT by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
• Target: AKT1, AKT2, AKT3
• Purity: AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody

Western Blot of Anti-AKT (RABBIT) Antibody. M: SuperSignal MW markers. Lane 1: rAKT1. Lane 2: rAKT2. Lane 3: rAKT3. Load: 50 ng per lane. Primary antibody: Anti-AKT (RABBIT) Antibody at 1:1000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:20000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.

Quick Database Links

Gene Symbol

AKT1, AKT2, AKT3

UniProt

P31749

RefSeq (Protein):NP_001014431.1