AKT1, AKT2, AKT3 Antibody

SKU: orb344703

Description

AKT antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: IHC: 1:500 - 1:2,000, IF: 1:500 - 1:2,000, WB: 1:1,000 - 1:5,000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  AKT Antibody is tested in western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Expect bands at ~56 kDa in size corresponding to AKT by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
• Target: AKT1, AKT2, AKT3
• Purity: AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody

Western Blot of Anti-AKT (RABBIT) Antibody. M: SuperSignal MW markers. Lane 1: rAKT1. Lane 2: rAKT2. Lane 3: rAKT3. Load: 50 ng per lane. Primary antibody: Anti-AKT (RABBIT) Antibody at 1:1000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:20000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.

Quick Database Links

Gene Symbol

AKT1, AKT2, AKT3

UniProt

P31749

RefSeq (Protein):NP_001014431.1