Agrimol B

SKU: orb1222041

Description

Agrimol B is a main active ingredient isolated from Agrimonia pilosa Ledeb.

Key Properties

• CAS Number: 55576-66-4
• MW: 682.76
• Purity: >98% (HPLC)
• Formula: C₃₇H₄₆O₁₂
• SMILES: CCCC(=O)C1=C(C(=C(C(=C1O)CC2=C(C(=C(C(=C2O)C(=O)C(C)CC)O)CC3=C(C(=C(C(=C3O)C)OC)C(=O)CCC)O)O)O)C)OC
• Target: NF-κB
• Solubility: In Vitro: DMSO : 5.56 mg/mL (8.14 mM)

Bioactivity

• In Vivo:
  Animal model: BALB/c nude mice (6-week-old, male, injected with PC-3 cells in 0.2 mL PBS subcutaneously). Dosage: 10 mg/kg. Administration: Orally, daily, after 15 days of cancer cell implantation. Result: Inhibited tumor growth in a mouse model of human prostate cancer, reduced the tumor volume at day 31 and day 32.
• In Vitro:
  Cell Cycle Analysis Cell line: PC-3 and A549 cells. Concentration: 29 and 63 μM (PC-3), 19 and 76 μM (A549). Incubation time: 10 min. Result: Dose-dependently increased cells at G0 in both cell lines. Reduced percentage of cells positive for Ki-67, and increased p27 positive cell population in PC-3 and A549 cells. Western blot analysis. Cell line: PC-3 and A549 cells. Concentration: 0, 29, 49, 63 μM (PC-3); 0, 19, 50, and 76 μM (A549). Incubation time: 3 days. Result: Reduced c-MYC, SKP2 and increased p27 in both cell lines, and down-regulated SPT16 and SSRP1 in A549 cells with no effect on CRM1 in both cell lines. Immunofluorescence Cell line: 3T3-L1 preadipocyte. Concentration: 0, 3, and 10 μM. Incubation time: 6 days. Result: Significantly increased nuclear positive rate of SIRT1; Markedly increased SIRT1 expression at 10 μM, the effect vanished at 3 μM. Down-regulated PPARγ and C/EBPα (CCAAT/enhancer-binding protein α) expression; Significantly decreased FAS (fatty acid synthesis), UCP-1 (uncoupling protein-1), and apoE (apolipoprotein E) expression at 10 μM.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

NF-κB