AEBSF hydrochloride

SKU: orb1300827

Description

AEBSF hydrochloride (Pefabloc SC) is an irreversible serine protease inhibitor effective against enzymes including trypsin, chymotrypsin, thrombin, plasmin, and kallikrein. It is widely used in vitro for protein biochemistry and cell biology research to prevent proteolytic degradation during sample preparation.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 30827-99-7
• MW: 239.69
• Purity: 98.92%
• Formula: C₈H₁₁ClFNO₂S
• SMILES: Cl.NCCc1ccc(cc1)S(F)(=O)=O
• Target: Influenza Virus,Thrombin,Serine Protease
• Solubility: H2O:24 mg/mL (100.13 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (20.86 mM);DMSO:247 mg/mL (1030.5 mM)

Bioactivity

• In Vivo:
  AEBSF is a serine protease inhibitor that suppresse the lysis of leukemia cells by macrophages without inhibitin the secretion of TNF-α and IL-1β by these cells. In five different human cell lines, AEBSF inhibit the formation of amyloid-beta Aβ) by blockin the secretion of both neural and non-neural β-secretase products. Additionally, AEBSF disrupts embryonic growth withi the endometrium and modifies protein secretion mechanisms to preven the adhesion of HeLa cells in human umbilical vein endothelial cells.
• In Vitro:
  In a mouse model of cockroach allergen-induced asthma, AEBSF was able to mitigate respiratory reactions and potential inflammation. Intraperitoneal injection of 76.8 mg/kg AEBSF daily in mice lethally inoculated with Toxoplasma gondii extended their survival period.
• Cell Research:
  The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into Each well of a 96-well microplate ( × 0^3 cells/200 μL well). After incubation for 24 h at 37℃, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/ml) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into Each well, and cells are cultured at 37℃n 5% CO2 for a Other 4 h. the media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nM.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Inhibitor, InfluenzaVirus, Influenza Virus, inhibit, AEBSF HCl, AEBSF hydrochloride, AEBSF Hydrochloride, AEBSF, Pefabloc SC, Serine Protease, Ser/Thr Protease, Serine endopeptidases, Serine proteases, SerineProtease, Thrombin, Threonine proteases

Compound Identifiers

PubChem CID

186136