AEBSF HCl

SKU: orb1300827

Description

AEBSF hydrochloride (Pefabloc SC) is an irreversible serine protease inhibitor effective against enzymes including trypsin, chymotrypsin, thrombin, plasmin, and kallikrein. It is widely used in vitro for protein biochemistry and cell biology research to prevent proteolytic degradation during sample preparation.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 30827-99-7
• MW: 239.69
• Purity: >98%
• Formula: C₈H₁₀FNO₂S HCl
• SMILES: Cl.NCCc1ccc(cc1)S(F)(=O)=O
• Target: Protease
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Water (up to 100 mg/ml).

Bioactivity

• In Vivo:
  AEBSF is a serine protease inhibitor that suppresses the lysis of leukemia cells by macrophages without inhibiting the secretion of TNF-α and IL-1β by these cells. In five different human cell lines, AEBSF inhibits the formation of amyloid-beta (Aβ) by blocking the secretion of both neural and non-neural β-secretase products. Additionally, AEBSF disrupts embryonic growth within the endometrium and modifies protein secretion mechanisms to prevent the adhesion of HeLa cells in human umbilical vein endothelial cells.
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• In Vitro:
  In a mouse model of cockroach allergen-induced asthma, AEBSF was able to mitigate respiratory reactions and potential inflammation. Intraperitoneal injection of 76.8 mg/kg AEBSF daily in mice lethally inoculated with Toxoplasma gondii extended their survival period.
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• Cell Research:
  The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, InfluenzaVirus, Influenza Virus, inhibit, AEBSF HCl, AEBSF hydrochloride, AEBSF Hydrochloride, AEBSF, Pefabloc SC, Serine Protease, Ser/Thr Protease, Serine endopeptidases, Serine proteases, SerineProtease, Thrombin, Threonine proteases

Compound Identifiers

PubChem CID

186136