Adenosine 5'-triphosphate disodium salt

SKU: orb1223480

Description

ATP Disodium salt is a P2 purinoceptor agonist.(In Vitro):ATP disodium salt (5mM; 1 hour) co-treatment with LPS (1 μg/ml) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs.ATP disodium salt (2 mM; 0.5-24 hours) induces secretion of interleukin 1β, KC and MIP-2 from bone marrow derived macrophages (BMDMs) in vitro in a caspase-1 activation-dependent manner.ATP disodium salt stimulats cytokine and chemokine secretion and inflammasome activation directly and indirectly induces in vitro neutrophil chemotaxis.(In Vivo):ATP disodium salt (50 mg/kg; i.p.) protects mice against bacterial infection in vivo.ATP disodium salt induces the secretion of IL1β, KC and MIP-2 and neutrophils recruitment in vivo.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 987-65-5
• MW: 551.14
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₄N₅O₁₃P₃·2Na
• SMILES: C1=NC2=C(C(=N1)N)N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)([O-])OP(=O)(O)[O-])O)O.[Na+].[Na+]
• Target: Others
• Solubility: DMSO: <1mg/ml;Water: 100mg/ml(181.44 mM);Ethanol: <1mg/ml

Bioactivity

• In Vivo:
  ATP disodium salt (50 mg/kg; i.p.) protects mice against bacterial infection In vivo. ATP disodium salt induces the secretion of IL1β, KC and MIP-2 and neutrophils recruitment In vivo. Animal model: Four-week-old Kunming mice (18-22 g). Dosage: 50 mg/kg. Administration: Intraperitoneal injection, before bacterial (E. coli) challenge. Result: Protected mice from bacterial infection.
• In Vitro:
  ATP disodium salt (5 mM; 1 hour) co-treatment with LPS (1 μg/ml) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs. ATP disodium salt (2 mM; 0.5-24 hours) induces secretion of interleukin 1β, KC and MIP-2 from bone marrow derived macrophages (BMDMs) in vitro in a caspase-1 activation-dependent manner. ATP disodium salt stimulats cytokine and chemokine secretion and inflammasome activation directly and indirectly induces in vitro neutrophil chemotaxis.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP | NSC 20268

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