Actein

SKU: orb1299995

Description

Actein is a research compound that stimulates osteoblast activity and protects against oxidative damage, showing potential for osteoporosis research. It also exhibits anti-proliferative effects in breast cancer cell studies by modulating calcium signaling and pathways like NF-κB and MEK.

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Key Properties

• CAS Number: 18642-44-9
• MW: 676.83
• Purity: ≥98%
• Formula: C₃₇H₅₆O₁₁
• SMILES: [H][C@]12C[C@@]3(C)[C@]4([H])CC[C@]5([H])[C@]6(C[C@@]46C[C@@H](OC(C)=O)[C@]3(C)[C@@]1([H])[C@H](C)C[C@]1(O[C@H](O)[C@@]3(C)O[C@@H]13)O2)CC[C@H](O[C@]1([H])OC[C@@H](O)[C@H](O)[C@H]1O)C5(C)C
• Target: Autophagy,JNK,Akt,ROS,Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (3.69 mM);DMSO:60 mg/mL (88.65 mM)

Bioactivity

• In Vitro:
  Actein, isolated from black cohosh, was subjected to in vitro experiments to investigate its functional bioactivities in osteoblastic MC3T3-E1 cells. METHODS AND RESULTS: Actein caused a significant elevation of alkaline phosphatase activity, collagen synthesis, osteocalcin production, mineralization, and glutathione content in the cells, suggesting that Actein has a stimulatory effect on osteoblastic bone formation or has potential activity against osteoporosis. We investigated the protective effects of Actein on mitochondrial electron transport inhibitor, antimycin A induced toxicity in osteoblastic MC3T3-E1 cells. Exposure of MC3T3-E1 cells to antimycin A caused significant decrease in cell viability and mineralization. However, pretreatment with Actein prior to antimycin A exposure significantly reduced antimycin A-induced cell damage by preventing mitochondrial membrane potential dissipation, complex IV inactivation, cardiolipin oxidation, ROS release, and nitrotyrosine increase, suggesting that Actein may be useful for protecting mitochondria against a burst of oxidative stress. In addition, Actein increased the phosphorylation of CREB (cAMP-response element-binding protein) inhibited by antimycin A and decreased the production of TNF-α induced by antimycin A. CONCLUSIONS:These findings suggest that Actein could prevent oxidative damage to osteoblasts in osteoporotic patients.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sodium Channel, PKB, Protein kinase B, TNF-α, Potassium Channel, CYP17, Apoptosis, ATPase, Autophagy, cAMP, Actein, Akt, Inhibitor, MEK, p53, inhibit, JNK, NF-κB

Compound Identifiers

PubChem CID

10032468