Acridine Orange base

SKU: orb1982091

Description

Acridine Orange base is a cell-permeable, nucleic acid-binding fluorescent dye. It differentially stains dsDNA (green, Ex/Em ~488/520 nm) and ssDNA/RNA (red, Ex/Em ~457/630 nm), enabling applications in cell cycle analysis, apoptosis detection, and viability assays in both in vitro and in vivo research models.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 494-38-2
• MW: 265.35
• Purity: 93.89%
• Formula: C₁₇H₁₉N₃
• SMILES: CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• Target: Others,Parasite
• Solubility: H2O:4 mg/mL (15.07 mM);DMSO:30 mg/mL (113.06 mM);1 M HCL:180 mg/mL (678.35 mM)

Bioactivity

• In Vitro:
  Fixed cell staining I. Sample preparation: 1. For cells in suspension culture or hematology samples: rinse cells once with ice-cold PBS and suspend in ice-cold PBS at 10^6 cells/mL. 2. For cells attached to tissue culture plates: collect cells from flasks or culture dishes by trypsinization, fill the trypsinized cells with cells floating in the culture medium (mainly isolated mitotic and dead cells), and then use culture medium containing serum to inactivate trypsin. Finally, suspend the cells in ice-cold PBS at about 10^6 cells/mL. 3. For cells isolated from solid tumors: rinse cells without any enzymes for cell dissociation and suspend in ice-cold PBS at about 10^6 cells/mL. II. Operation steps: 1. Transfer 1 mL of cell suspension with a Pasteur pipette to a 15 mL conical glass tube containing 10 mL of ice-cold 70% ethanol. Fix the cells on ice for ≥2 hours. 2. Centrifuge the tube at 300 × g, 4°C for 5 min. Remove all ethanol with ice-cold PBS, rinse once, and suspend in ice-cold PBS at a density of <2×10^6 cells/mL. 3. Take 0.2 mL of cell suspension (≤2×10^5 cells) and transfer to a small tube. Cool on ice. 4. Add 0.4 mL of ice-cold permeabilization solution. Wait 15 seconds and keep cells on ice. 5. Add 1.2 mL of ice-cold AO staining solution. Keep cells on ice. 6. Add 1.2 mL of ice-cold Acridine Orange base staining solution. Keep cells on ice. 7.After adding Acridine Orange base solution, measure and record cell fluorescence using a flow cytometer within 2 to 10 minutes.

Storage & Handling

• Storage: keep away from direct sunlight | store at 4°C | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

62344