Acetylcholine iodide

SKU: orb1300499

Description

Acetylcholine iodide is a key cholinergic neurotransmitter essential for neuromuscular, autonomic, and central nervous system signaling. It is widely used in research to study receptor function, synaptic transmission, and cholinergic pathways in both in vitro assays and in vivo physiological models.

Research Area

Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 2260-50-6
• MW: 273.11
• Purity: 99.86% (May vary between batches)
• Formula: C₇H₁₆NO₂·I
• SMILES: CC(=O)OCC[N+](C)(C)C.[I-]
• Target: Others,Endogenous Metabolite,AChR
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.32 mM);DMSO:50 mg/mL (183.08 mM);H2O:33 mg/mL (120.83 mM)

Bioactivity

• In Vivo:
  Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury.
• In Vitro:
  Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells.
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• Cell Research:
  The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Acetylcholine iodide, Acetylcholine Iodide, Acetylcholine, Acetylcolina, ACh

Compound Identifiers

PubChem CID

75271