H3K14ac Antibody

SKU: orb1256504

Description

H3K14ac Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:50 - 1:100CHIP seq: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3
• Target: H3K14ac
• Molecular Weight: Observed: 15kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H3F3A, H3t, H3.4, H3/g, H3FT

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K14 antibody (orb1256504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human stomach using Acetyl-Histone H3-K14 antibody (orb1256504) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K14 antibody (orb1256504) at dilution of 1:100. HeLa cells were treated by TSA (1uM) for 18 hours (left). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

H3K14ac

UniProt

Q16695