Acetaminophen

SKU: orb1310976

Description

Acetaminophen

Research Area

Epigenetics & Chromatin, Immunology & Inflammation, Metabolism Research, Neuroscience

Key Properties

• CAS Number: 103-90-2
• MW: 151.1626
• Purity: >99.99% (May vary between batches)
• Formula: C₈H₉NO₂
• SMILES: CC(=O)NC1=CC=C(O)C=C1
• Target: Histone Acetyltransferase,COX,Endogenous Metabolite
• Solubility: Ethanol:15.1 mg/mL (99.89 mM);H2O:20 mg/mL (132.31 mM);DMSO:237 mg/mL (1567.88 mM)

Bioactivity

• Target IC50:
  HepG2 cells:108.6 µg/ml|COX-2:25.8 μM|COX-1:113.7 μM|MCF-7 cells:108.4 µg/ml|COX-3:64 μM
• In Vivo:
  METHODS: To detect hepatotoxicity in vivo, Acetaminophen was administered intraperitoneally to mice (300 mg/kg) and rats (1 g/kg). RESULTS: Extensive liver necrosis was observed in mice, but little damage was observed in rat samples. The rats were highly resistant to Acetaminophen-induced liver injury. METHODS: To test for in vivo hepatotoxicity, Acetaminophen was administered to aged and weakened mice acutely (300 mg/kg by gavage), chronically (100 mg/kg by diet once daily for six weeks), or subacutely (250 mg/kg by gavage three times daily for three days). RESULTS: There was no overall increase in Acetaminophen hepatotoxicity with age or frailty in mice, despite changes in certain pathways that would be expected to affect susceptibility to Acetaminophen toxicity.
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• In Vitro:
  METHODS: Non-melanoma and melanoma cell lines were treated with Acetaminophen (100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Acetaminophen showed considerable toxicity in melanoma cell lines SK-MEL-5, MeWo, B16-F0 and B16-F10, resulting in 40±3%, 45±7%, 66±8% and 60±5% cytotoxicity, respectively. Acetaminophen showed negligible toxicity at 100 μM in the non-melanoma cell lines PC-3, BJ, Saos-2 and SW-620 cells. METHODS: Neuroblastoma cells SH-SY5Y were treated with Acetaminophen (2 mM) for 24-48 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Acetaminophen induced cytochrome c release from mitochondria in a time-dependent manner, reaching a maximum level after 48 h of treatment. In addition, immunoblot analysis of cytoplasmic and mitochondrial fractions showed that Acetaminophen was able to induce the accumulation of Bax into mitochondria at 24 h after treatment.
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• Cell Research:
  Cells are exposed to Acetaminophen for 48 hours. Cell viability is determined by the trypan blue exclusion method. Intracellular GSH is measured by recording the disulfide, GS-TNB and 5-thio-nitrobenzoic acid (TNB), the yellow colored compound formed by the reaction between GSH with DTNB.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclooxygenase, COX-2, COX, COX-1, Acetaminophen, 4-Acetamidophenol, 4'-Hydroxyacetanilide, APAP, inhibit, Paracetamol, Inhibitor, HATs, HAT, Histone Acetyltransferase, HistoneAcetyltransferase, EndogenousMetabolite, Endogenous Metabolite

Compound Identifiers

PubChem CID

1983