Ac-DEVD-AMC

SKU: orb1307235

Description

Ac-DEVD-AMC is a fluorogenic peptide substrate specifically cleaved by caspase-3. It is widely used in vitro to measure caspase-3 activity during apoptosis research in fields like neurobiology and cancer studies, facilitating the quantification of programmed cell death.

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 169332-61-0
• MW: 675.64
• Purity: 98.20%
• Formula: C₃₀H₃₇N₅O₁₃
• SMILES: CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc2c(C)cc(=O)oc2c1
• Target: Caspase
• Solubility: H2O:< 0.1 mg/mL (insoluble);DMSO:≤ 10 mg/mL (14.80 mM)

Bioactivity

• In Vitro:
  The AMC moiety is highest at 2 h with an area of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small . Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity.
• Cell Research:
  Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety .

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AcDEVDAMC, Ac-DEVD-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN, AC-ASP-MET-GLN-ASP-AMC, Ac DEVD AMC, Caspase-3, Caspase, Inhibitor, inhibit

Compound Identifiers

PubChem CID

9896164