A77-01

SKU: orb1224483

Description

A potent inhibitor of TGF-β type I receptor superfamily activin-like kinase ALK5 with IC50 of 25 nM; A-77-01 is a close analogue of A-83-01(Axon 1421) and has a very similar biological profile of A-83-01.

Key Properties

• CAS Number: 607737-87-1
• MW: 286.3306
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₄N₄
• SMILES: CC1=CC=CC(=N1)C2=C(C=NN2)C3=CC=NC4=CC=CC=C34
• Target: TGFBR
• Solubility: 10 mM in DMSO

Bioactivity

• In Vitro:
  A-77-01 (0.01-10 μM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion. A-77-01 (1 μM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β. A-77-01 (1 μM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2. RT-PCR Cell line: Mv1Lu cells. Concentration: 0.01 μM, 0.03 μM, 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM Incubation time: 25 hours. Result: Inhibited the luciferase activity induced by TGF-β in concentration-dependent fashion. Western blot analysis. Cell line: HaCaT cells. Concentration: 1 μM. Incubation time: 30 minutes. Result: Inhibited TGF-β-induced phosphorylation of Smad2.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A77-01 | A-77-01

Quick Database Links

Gene Symbol

TGFBR