8-Azido-ATP

• Catalog Number: orb1734041
• Category: Tools
• Description: 8-Azido-ATP
• Form/Appearance: solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Concentration: 10 mM-11 mM
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 548.19 g/mol (free acid); Detected MW: 548.00 g/mol (free acid)
• Application notes: Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP). Identification of protein ATP-binding sites. The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively); to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively); to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. < br>< b>Spectroscopic Propertie: λmax 281 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Formula: C₁₀H₁₅N₈O₁₃P₃
• SMILES: Nc1c2c(n(c(n2)N=[N+]=[N-])[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)ncn1
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only