7,8-Dihydroxyflavone

SKU: orb1304985

Description

7,8-Dihydroxyflavone

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 38183-03-8
• MW: 254.24
• Purity: 99.81%
• Formula: C₁₅H₁₀O₄
• SMILES: Oc1ccc2c(oc(cc2=O)-c2ccccc2)c1O
• Target: Apoptosis,Trk receptor
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:250 mg/mL (983.32 mM);Ethanol:1 mg/mL (3.93 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.87 mM)

Bioactivity

• Target IC50:
  TrkB receptor:320 nM(Kd)
• In Vivo:
  7,8-Dihydroxyflavone is a bioavailable chemical that can pass throug the BBB to provoke TrkB and its downstream PI3K/Akt and MAPK activation in mouse brain upon intraperitoneal or oral administration. 7,8-DHF promote the survival and reduces apoptosis in cortical neurons of traumatic brain injury as administration of 7,8-DHF at 3 h post-injury reduces brain tissue damage vi the PI3K/Akt pathway. Its treatment does not induce any apparent toxicity in mice and is not toxic to the mice durin the chronic treatment. 7,8-DHF displays robust therapeutic efficacy toward Alzheimer's disease and inhibits obesity through activating muscular Trk.
• In Vitro:
  7,8-DHF is one o the positive compounds that specifically activate TrkB, but not TrkA or TrkC, at a concentration of 250 nM. In addition to cortical and hippocampal neurons, 7,8-DHF also protects Other cell types includin the RGC (retinal ganglion cells) and PC12 cells from excitotoxic and oxidative stress-induced apoptosis and cell death. Thus, it has neur Protective propertie.
• Cell Research:
  PC12 cells are seeded in 96-well plates at 104/well. After pretreatment with 7,8-DHF (1-25 μM) for 1 h the cells are exposed to 6-OHDA (100 μM) for subsequent 24 h the PI3k inhibitor LY294002 or MEK inhibitor PD98059 is added 30 min before 7,8-DHF treatment. At the end of the experiment, PC12 cells are incubated with 20 μl of MTT solution (5 mg mL in PBS) for 4 h at 37℃he dark blue formazan product due to the reduction of MTT is dissolved in 150 μl of DMSO, an the absorbance at 570 nM is recorded with a microplate reader the viability is expressed a the percentage o the untreated control cells. (Only for Reference)

Storage & Handling

• Storage: keep away from moisture,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, 7,8-DHF, 7,8Dihydroxyflavone, 7,8-Dihydroxyflavone, 7,8 Dihydroxyflavone, Apoptosis, Tropomyosin related kinase receptor, Trkreceptor, TrkB, Trk receptor, Trk Receptor

Compound Identifiers

PubChem CID

1880