7,4'-Di-O-methylapigenin

SKU: orb1301580

Description

7,4'-Di-O-methylapigenin is a bioactive flavone that inhibits α-glucosidase and α-amylase, suggesting antidiabetic potential. It enhances glucose uptake in L6 myotubes and exhibits a hypolipidemic effect in 3T3-L1 pre-adipocytes, supporting research into metabolic disorders.

Research Area

Metabolism Research

Key Properties

• CAS Number: 5128-44-9
• MW: 298.29
• Purity: 99.40%
• Formula: C₁₇H₁₄O₅
• SMILES: COc1ccc(cc1)-c1cc(=O)c2c(O)cc(OC)cc2o1
• Target: Endogenous Metabolite,Amylase
• Solubility: DMSO:7 mg/mL (23.47 mM)

Bioactivity

• In Vitro:
  Antioxidant activity in terms of TRP (196.03 μg/mg GAE), DPPH assay (IC50-173.09 μg/mL), hydroxyl radical scavenging assay (IC50-20.42 μg/mL), inhibiting LDL oxidation (IC50-51.99 μg/mL) and to enhance SOD activity (25.3 %) was higher in ethyl acetate extract (EAP). Phenolic and flavonoid content was measured and showed a positive correlation with antioxidant activity. Presence of 4',7-DIMETHOXY-5-HYDROXYFLAVONE and piperine (Pip) in EAP was determined by HPTLC analysis and was isolated. 4',7-DIMETHOXY-5-HYDROXYFLAVONE inhibited α-glucosidase and α-amylase enzymes and enhanced 2-NBDG uptake in L6 cells. Hypolipidemic effect of 4',7-DIMETHOXY-5-HYDROXYFLAVONE on mouse pre-adipocyte (3T3L1) cell lines also showed a dose dependent reduction on lipid droplet content and effective concentration range was determined as 1-2.5 μg/mL. Suggested that Piper longum fruits can provide a natural source of antioxidants with antidiabetic and anti obesity potential.
• Cell Research:
  Solvent extracts from Piper longum fruits using hexane, ethyl acetate, methanol, 70 % methanol-water were taken and 4',7-DIMETHOXY-5-HYDROXYFLAVONE was isolated from ethyl acetate extract. Antioxidant activity, glucose uptake potential and adipocyte differentiation assay was performed with extract and pure compound.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

α-glucosidase, α-Amylase, inhibit, Inhibitor, 4',7-DIMETHOXY-5-HYDROXYFLAVONE, 7,4'DiOmethylapigenin, 7,4'-Di-O-methylapigenin, 7,4' Di O methylapigenin

Compound Identifiers

PubChem CID

5281601