7-Epi 10-desacetyl paclitaxel

SKU: orb1301537

Description

7-Epi 10-desacetyl paclitaxel (10-Deacetyl-7-epipaclitaxel) is a potent microtubule-stabilizing agent, exhibiting an IC50 of 0.085 nM against HeLa cervical cancer cells in vitro. This taxane derivative is a valuable research tool for studying mechanisms of cytotoxicity and resistance in cancer biology, with applications in both cellular and biochemical assays.

Key Properties

• CAS Number: 78454-17-8
• MW: 811.87
• Purity: 99.01% (May vary between batches)
• Formula: C₄₅H₄₉NO₁₃
• SMILES: [H][C@@]12C[C@@H](O)[C@@]3(C)C(=O)[C@H](O)C4=C(C)[C@H](C[C@@](O)([C@@H](OC(=O)c5ccccc5)[C@]3([H])[C@@]1(CO2)OC(C)=O)C4(C)C)OC(=O)[C@H](O)[C@@H](NC(=O)c1ccccc1)c1ccccc1
• Target: ADC Cytotoxin
• Solubility: DMSO:8.12 mg/mL (10 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (1.23 mM)

Bioactivity

• In Vitro:
  7-Epi-10-deacetyltaxol(EDT) exhibited significant in vitro cytotoxicity in HepG2 cells.Nuclear condensation and DNA fragmentation were observed in cells treated with fungal EDT. Besides, the fungal EDT arrested HepG2 cells at G2/M phase of cell cycle. Furthermore, fungal EDT induced apoptosis in HepG2 cells in a dose-dependent manner associated with ROS generation and increased Bax/Bcl-2 ratio, p38 MAPKs and PARP cleavage.
• Cell Research:
  The endophytic fungus was identified by traditional and molecular taxonomical characterization and the fungal 7-Epi-10-deacetyltaxol(EDT) was purified using column chromatography and confirmed by various spectroscopic and chromatographic comparisons with authentic paclitaxel. Studied the in vitro effects of EDT on HepG2 cells for parameters such as cell cycle distribution, DNA fragmentation, reactive oxygen species (ROS) generation and nuclear morphology. Further, western blot analysis was used to evaluate Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), p38-mitogen activated protein kinase (MAPK) and poly [ADP-ribose] polymerase (PARP) expression.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADC Cytotoxin, ADCCytotoxin, 7-Epi 10-desacetyl paclitaxel, 7Epi 10desacetyl paclitaxel, 7-Epi-10-deacetyltaxol, 7 Epi 10 desacetyl paclitaxel, 10-Deacetyl-7-epipaclitaxel, Inhibitor, inhibit

Compound Identifiers

PubChem CID

14355298