6RK73

SKU: orb1710529

Description

6RK73 is a potent, covalent, and irreversible inhibitor that selectively targets UCHL1 with an IC50 of 0.23 µM, demonstrating over 1000-fold selectivity against UCHL3. This small molecule is a valuable chemical probe for studying deubiquitinase function in neurodegenerative disease models and cancer research, both in vitro and in vivo.

Research Area

Cell Biology, Molecular Biology, Protein Biochemistry

Key Properties

• CAS Number: 1895050-66-4
• MW: 307.37
• Purity: >99.99% (May vary between batches)
• Formula: C₁₃H₁₇N₅O₂S
• SMILES: O=C(Nc1ncc(s1)N1CCOCC1)[C@H]1CCN(C1)C#N
• Target: DUB,Others
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (13.01 mM);DMSO:112.5 mg/mL (366.01 mM)

Bioactivity

• Target IC50:
  UCHL3:236 µM |UCHL1:0.23 µM
• In Vivo:
  6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish.
• In Vitro:
  6RK73 (5 µM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells. 6RK73 (5 µM; 24-48 hours) results in migration significantly slower than the DMSO control group in MDA-MB-436 cells.
• Cell Research:
  Cell Line: MDA-MB-436 cells Concentration: 5 µM Incubation Time: 24, 48 hours

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6RK73, 6RK-73

Compound Identifiers

PubChem CID

121248200