6-FAM

SKU: orb1307239

Description

6-FAM

Key Properties

• CAS Number: 3301-79-9
• MW: 376.32
• Purity: 98.73%
• Formula: C₂₁H₁₂O₇
• SMILES: OC(=O)c1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31
• Target: Others
• Solubility: DMSO:40 mg/mL (106.29 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2 mg/mL (5.31 mM)

Bioactivity

• In Vitro:
  6-FAM labeled oligonucleotide probes for fluorescence in situ hybridization (FISH) I. Solution preparation: 1. Preparation of stock solution: Dissolve 6-FAM in DMSO or ethanol to prepare a 1-10 mM stock solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) 2. Preparation of working solution: Dilut the 6-FAM stock solution with PBS or Other suitable buffer, usually 1-10 µM; (Selec the appropriate working solution concentration according to Experimental requirements, and prepare it for immediate use) II. Operation steps: 1. Sample preparation: Prepare chromosome Samples of chicken embryonic cells, including colchicine treatment, hypotonic swelling and methanol-glacial acetic acid fixation. 2. Pretreatment: Chromosomes are treated with RNase A, pepsin and formaldehyde. 3. Hybridization: 6-FAM labeled oligonucleotides were dissolved as probes in hybridization buffer (50% formamide, × SC, 10% dextran sulfate) containing E. coli tRNA at a concentration of 20 ng/μL. After co-denaturation of DNA and probe, hybridization was performed overnight at room temperature in a humidified box; 4. Washing and counterstaining: After hybridization the slides were washed three times in × SC, dehydrated in a series of 70%-96% ethanol, and sealed with an antifade containing DAP I after drying; 5. Observation and analysis the Samples were observed using a Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters, an the chromosome color images were captured and processed using QFISH software.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, 6FAM, 6-FAM, 6-Carboxyfluorescein, 6 FAM

Compound Identifiers

PubChem CID

76806