5-Vinyl-uridine (5-VU)

SKU: orb532300

Description

5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring. The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  RNA synthesis monitoring (potentially). ; Spectroscopic Propertie: max 288 nm, 8.5 L mmol-1 cm-1, (Methanol).

Key Properties

• CAS Number: 55520-64-4
• MW: Theoretical MW: 270.24 g/mol; Detected MW: 270.09 g/mol
• Purity: 98% (HPLC)
• Formula: C₁₁H₁₄N₂O₆
• SMILES: n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only