5-Propargylamino-dCTP-Texas Red

• Catalog Number: orb533144
• Category: Tools
• Description: 5-Propargylamino-dCTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluo-rescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-TexasRed/dCTP ratio for PCR and Nick Translation: 20-30% Propargylamino-dCTP-TexasRed7/80-70% dCTP (PCR), 30-50% Propargylamino-dCTP-TexasRed/70-50% dCTP (Nick Translation).
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: solution in water; Color: red-violet; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1222.07 g/mol (free acid); Detected MW: 1221.24 g/mol (free acid)
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNS(=O)(=O)c1cc(c(cc1)C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-])N)O.[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNS(=O)(=O)c1cc(ccc1C1=c2c(Oc3c1cc1c4c3CCCN4CCC1)c1c3=[N+](CCCc3c2)CCC1)S(=O)(=O)[O-])N)O
• Formula: C₄₉H₅₈N₇O₂₀P₃S₂
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 588 nm, λem 609 nm, ε 80.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.