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Catalog Number | orb533139 |
---|---|
Category | Tools |
Description | 5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/70-50% dCTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in water; Color: blue; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 1148.08 g/mol (free acid); Detected MW: 1147.40 g/mol (free acid) |
SMILES | [C@@H]1(CC(OC1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCN(C(=O)c1c(C2=c3cc4c5=[N+](CCC4)CCCc5c3C(c3c2cc2c(c3)N(C(CC2C)(C)C)CC)(C)C)cccc1)C)N)O |
Formula | C54H68N7O15P3 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Spectroscopic Propertie: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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