4-O-Methyl honokiol

SKU: orb1710555

Description

4-O-Methyl honokiol

Research Area

Metabolism Research, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 68592-15-4
• MW: 280.36
• Purity: 99.31%
• Formula: C₁₉H₂₀O₂
• SMILES: COc1ccc(cc1CC=C)-c1cc(CC=C)ccc1O
• Target: PPAR,NF-κB
• Solubility: DMSO:80 mg/mL (285.35 mM)

Bioactivity

• In Vivo:
  4-O-Methyl honokiol (40 or 80 mg/kg; i.p.; every day for 4 weeks) inhibits the growth of SW620 and PC3 tumors in the SW620 and PC3 xenograft model. 4-O-Methyl honokiol significantly increases the expression of p21 and PPARγ in the tumor tissues. 4-O-Methyl honokiol (0.5 or 1 mg/kg; every day for 3 weeks) significantly ameliorates LPS-induced memory impairment and inhibits LPS-induced iNOS and COX-2 expression in mice. 4-O-Methyl honokiol also shows inhibitory activities against the Aβ1-42 accumulation and activates astrocytes and microglia in LPS-injected mice brains.
• In Vitro:
  4-O-Methyl honokiol (20 μM) increases the expression, transcription, DNA binding activities, and nuclear translocation of PPARγ in both prostate PC-3 and LNCap cells. 4-O-Methyl honokiol (0-30 μM) inhibits LNCaP and PC-3 cancer cell growth, causes G0/G1 phase arrest, and induces apoptotic cell death, and such effects can be reversed by PPARγ antagonist. 4-O-Methyl honokiol inhibits NF-κB activity and cancer cell growth, but such effects as well as its activation of PPARγ can be abolished by knockdown of p21. 4-O-methyl honokiol (0.5, 1, and 2 μM) reduces LPS-induced release of NO, PGE2, ROS, TNF-α, and IL-1β in cultured astrocytes, and amyloidogenesis in cultured astrocytes and microglial BV-2 cells.
• Cell Research:
  Cells (5 × 10^4 cells per well) are plated onto 24-well plates. The cell growth inhibitory effect of 4-O-Methyl honokiol is evaluated in cells treated with 4-O-Methyl honokiol (0-30 μM) for 0-72 h, using an excluded trypan blue assay .
• Animal Research:
  Six-week-old male BALB/c athymic nude mice are used in the assay. SW620 and PC3 cells are injected s.c. (1 × 10^7 cells in 0.1 mL PBS per animal) into the lower right flanks of mice. After 20 days, when the tumors have reached an average volume of 300-400 mm3 or about 50 mm3, the tumor-bearing nude mice are i.p. injected with 4-O-Methyl honokiol (40 and 80 mg/kg dissolved in 0.1% DMSO) twice per week for 3 weeks. Cisplatin (10 mg/kg) is also i.p. injected once a week as a positive control. The group treated with 0.1% DMSO is designated as the control. The tumor volumes are measured with vernier calipers and calculated by the following formula: (A × B^2)/2, where A is the larger and B is the smaller of the two dimensions .

Storage & Handling

• Storage: store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPARγ, NF-κB, NFκB, NF-kB, NFkB, 4 O Methyl honokiol, 4-O-Methylhonokiol, 4OMethyl honokiol, 4-O-Methyl honokiol

Compound Identifiers

PubChem CID

155160