4-Hydroxyisoleucine

SKU: orb1220410

Description

4-Hydroxyisoleucine has antidepressant-like antidyslipidemic and antihyperglycemic effects. It displays an insulinotropic activity.

Key Properties

• CAS Number: 781658-23-9
• MW: 147.17
• Purity: >98% (HPLC)
• Formula: C₆H₁₃NO₃
• SMILES: CC(O)[C@H](C)[C@H](N)C(=O)O
• Target: Others
• Solubility: In Vitro: H2O : 100 mg/mL (679.49 mM)

Bioactivity

• In Vivo:
  Animal model: Fructose-fed rat. Dosage: 50 mg/kg. Administration: Oral gavage (p.o.), daily, 8 weeks. Result: Decreased the levels of glucose and ALT. Reduced 80% of fructosehe-induced AST release to 151 ± 45 U/mL. Animal model: Type 2 diabetic rat. Dosage: 50 mg/kg. Administration: i. g. , daily, 14 days. Result: Restored the level of HDL-cholesterol to levels comparable to controls. Animal model: Male C57BL/6 mice. Dosage: 50-200 mg/kg. Administration: Oral gavage (p.o.), 8 weeks. Result: Decreases the body weights of mice in a dose-dependent manner. Decreased blood glucose levels and fasting plasma insulin content in mice. Decreased the expression of TLR4, inhibited the phosphorylation of JNK, and increased the production of IκB-α. Animal model: Type 1 diabetic rat. Dosage: 50 mg/kg. Administration: i. g. , daily, 14 days. Result: Improved appearance and heavy ocular vascularization. Reduced the blood glucose from 500 mg/dl to 330 mg/dl. Decreased the levels of lipid markers (TG, LDL and HDL) and uric acid.
• In Vitro:
  Cell Viability Assay Cell line: RAW264.7 macrophages and 3 T3-L1 adipocytes Concentration: 0-20 μM. Incubation time: 6 h. Result: Didn’t affect the cell viability. Cell Migration Assay Cell line: RAW264.7 macrophages and 3 T3-L1 adipocytes Concentration: 20 μM. Incubation time: 24 h. Result: Inhibited the migration of RAW264.7 macrophages in 3 T3-L1 adipocytes. Western blot analysis. Cell line: L6-GLUT4myc. Concentration: 25 μM. Incubation time: 16 h. Result: Increased the AKT (Ser-473) phosphorylation.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-?Hydroxy-?L-?isoleucine

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Gene Symbol

Others