3BDO

SKU: orb1308724

Description

3BDO is a novel small molecule activator of mTOR, functioning as an autophagy inhibitor. It is utilized in research exploring mTOR signaling pathways, cellular metabolism, and autophagy-related processes in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 890405-51-3
• MW: 327.33
• Purity: 97.76%
• Formula: C₁₈H₁₇NO₅
• SMILES: [O-][N+](=O)c1ccccc1OCC1CC(Cc2ccccc2)C(=O)O1
• Target: Autophagy,mTOR,Apoptosis
• Solubility: DMSO:150 mg/mL (458.25 mM);Ethanol:20 mg/mL (61.1 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (12.22 mM)

Bioactivity

• In Vivo:
  Immunofluorescence assay demonstrates that treatment with 3BDO enhances p-p70S6K levels while reducing ATG13 protein levels in the plaque endothelium of mice, without affecting the phosphorylation of mTOR's immediate downstream targets p70S6K and 4EBP1. Comparatively, in apoE-/- mice, 3BDO treatment notably inhibits endothelium autophagy and apoptosis, thereby offering protection against endothelial injury in atherosclerosis. Furthermore, 3BDO treatment contributes to the stabilization of established atherosclerotic lesions in apoE-/- mice and results in a significant reduction in the serum levels of IL-6 and IL-8.
• In Vitro:
  Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced by rapamycin and inhibits its effect in HUVECs. Phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses this phosphorylation. These results demonstrate that 3BDO suppresses the increased MAP1LC3B puncta, MAP1LC3B-II levels, and decreased SQSTM1 protein levels induced by rapamycin. Additionally, 3BDO decreases FLJ11812 levels in HUVECs in a dose- and time-dependent manner, while overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

mTOR, inhibit, Inhibitor, Mammalian target of Rapamycin, Apoptosis, Autophagy, 3BDO

Compound Identifiers

PubChem CID

16216349