3,6-Dihydroxyflavone

SKU: orb1299327

Description

This small molecule inhibitor of the Notch signaling pathway suppresses epithelial-mesenchymal transition (EMT) in breast cancer cells. It is a useful research tool for studying oncogenic signaling, metastasis, and potential therapeutic strategies in both in vitro and in vivo cancer models.

Research Area

Cell Biology

Key Properties

• CAS Number: 108238-41-1
• MW: 254.24
• Purity: >98%
• Formula: C₁₅H₁₀O₄
• SMILES: Oc1ccc2oc(-c3ccccc3)c(O)c(=O)c2c1
• Target: Apoptosis,Others
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.87 mM);DMSO:27.5 mg/mL (108.17 mM)

Bioactivity

• In Vitro:
  3,6-Dihydroxyflavone(3,6-DHF) could effectively inhibit EMT in BC cells in vitro and in vivo. 3,6-DHF effectively inhibits the formation and proliferation of BCSCs, and consequently reduces the tumor-initiating capacity of tumor cells in NOD/SCID mice. Optical in vivo imaging of cancer metastasis showed that 3,6-DHF administration suppresses the lung metastasis of BC cells in vivo. Further studies indicated that 3,6-DHF down-regulates Notch1, NICD, Hes-1 and c-Myc, consequently decreasing the formation of the functional transcriptional unit of NICD-CSL-MAML, causing Notch signaling inactivation in BC cells. Over-expression of Notch1 or inhibition of miR-34a significantly reduced the inhibitory effects of 3,6-DHF on EMT, CSCs, as well as cells migration and invasion in BC cells.
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• Cell Research:
  Cells were cultured in six-well plates until they reached 100% confluence. A vertical or horizontal wound was gently created in monolayers using a 20 μl sterile pipette tip. The cells were then washed for 3 times with growth medium to remove the detached cells, and the medium was added with fresh medium treated with 3,6-DHF. Images were captured using an inverted microscope and camera at designed times to assess the inhibition of wound closure.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

lipid peroxidation, Inhibitor, inhibit, PARP, Apoptosis, caspase cascade, 3,6 Dihydroxyflavone, 3,6Dihydroxyflavone, 3,6-Dihydroxyflavone, 3,6-DHF, cell viability, oxidative stress

Compound Identifiers

PubChem CID

688659