3'-O-(2-Nitrobenzyl)-dTTP

SKU: orb3218415

Description

3'-O-(2-Nitrobenzyl)-dTTP serves as a reversible terminator for DNA chain extension. It interacts with the 9°N polymerase (exo-) A485L/Y409V, being recognized and incorporated into the extending DNA chain, temporarily halting polymerase activity. This compound features a 3'-O-(2-nitrobenzyl) blocking group, which can be removed by laser irradiation to regenerate the free 3'-OH group and restore polymerase function. 3'-O-(2-Nitrobenzyl)-dTTP is applicable in DNA sequencing research.

Key Properties

• CAS Number: 219607-49-5
• MW: 617.29
• Formula: C₁₇H₂₂N₃O₁₆P₃
• SMILES: O=C1N([C@@H]2O[C@H](COP(OP(OP(=O)(O)O)(=O)O)(=O)O)[C@@H](OCC3=C(N(=O)=O)C=CC=C3)C2)C=C(C)C(=O)N1
• Target: DNA/RNA Synthesis

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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Gene Symbol

DNA/RNA Synthesis