3-methyl toxoflavin

SKU: orb1220950

Description

3-methyltoxoflavin is an effective inhibitor of protein disulfide isomerase (PDI) and its IC50 value is 170 nM.

Key Properties

• CAS Number: 32502-62-8
• MW: 207.19
• Purity: >98% (HPLC)
• Formula: C₈H₉N₅O₂
• SMILES: Cc1nn(C)c2nc(=O)n(C)c(=O)c2n1
• Target: protein disulfide isomerases
• Solubility: DMSO:150 mg/mL (723.97 mM)

Bioactivity

• In Vitro:
  3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM. 3-Methyltoxoflavin is toxic in a panel of human glioblastoma cell lines. From the screen, 3-Methyltoxoflavin emerges as the most cytotoxic inhibitor of PDI. Bromouridine labeling and sequencing (Bru-seq) of nascent RNA reveals that 3-Methyltoxoflavin induces Nrf2 antioxidant response, ER stress response, and autophagy. Specifically, 3-Methyltoxoflavin upregulates heme oxygenase 1 and SLC7A11 transcription and protein expression and represses PDI target genes such as TXNIP and EGR1. Interestingly, 3-Methyltoxoflavin-induced cell death does not proceed via apoptosis or necrosis, but by a mixture of autophagy and ferroptosis.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3-methyltoxoflavin

Quick Database Links

Gene Symbol

protein disulfide isomerases