3-Hydroxybutyric acid

SKU: orb1302428

Description

Butanoic acid is an endogenous HDAC inhibitor with IC50 values of 2.4 mM, 4.5 mM, and 5.3 mM for HDAC3, HDAC4, and HDAC1, respectively. Its metabolite, 3-hydroxybutyric acid, is studied for roles in energy metabolism, neuroprotection, and insulin resistance in metabolic and neurological research models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 300-85-6
• MW: 104.1
• Purity: 99.77% (May vary between batches)
• Formula: C₄H₈O₃
• SMILES: C(C(C)O)C(O)=O
• Target: Endogenous Metabolite
• Solubility: H2O:200 mg/mL (1921.23 mM);DMSO:262.5 mg/mL (2521.61 mM)

Bioactivity

• Target IC50:
  HDAC3:2.4 mM|HDAC1:5.3 mM|HDAC4:4.5 mM
• In Vivo:
  METHODS: To study the neuroprotective effect of 3-Hydroxybutyric acid, 3-Hydroxybutyric acid (2 mM or 5 mM) was injected intraventricular into C57BL/6 mice. RESULTS: 3-Hydroxybutyric acid reduces the binding of HDAC2 and HDAC3 to the pI promoter of _Bdnf_. METHODS: To study the neuroprotective effect of 3-Hydroxybutyric acid, 3-Hydroxybutyric acid (5.0 mmol/kg) was intraperitoneally injected into neonatal mice after hypoxia-ischemia treatment. RESULTS: 3-Hydroxybutyric acid reduces the number of Tunel-positive cells in brain regions, reduces cerebral infarction, and thereby alleviates brain injury.
• In Vitro:
  METHODS: After HEK293 cells were treated with 3-Hydroxybutyric acid (10 mM) for 24 hours, the acetylation levels of histone H3K9 on the _Foxo3a_ and _Mt2_ promoters were detected. RESULTS: 3-Hydroxybutyric acid increases the acetylation of histone H3K9 in HEK293 cells on the _Foxo3a_ and _Mt2_ promoters. METHODS: Mouse glial cells were treated with 3-Hydroxybutyric acid (10 mM) for 48 hours, and the cell proliferation was detected by the MTT assay. RESULTS: 3-Hydroxybutyric acid significantly promotes cell proliferation. METHODS: Human monocytes were treated with 3-Hydroxybutyric acid (1-20 mM) for 24 hours, and the protein levels were detected by Western Blot. RESULTS: 3-Hydroxybutyric acid dose-dependently inhibits the production of IL-1β and IL-18 mediated by the NLRP3 inflammasome.

Storage & Handling

• Storage: store at low temperature,store under nitrogen,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3Hydroxybutyric acid, 3-Hydroxybutyric acid, 3 Hydroxybutyric acid, Butanoic acid, Endogenous Metabolite, EndogenousMetabolite, diabetes, metabolite, Inhibitor, lipids, inhibit, membrane, Human Endogenous Metabolite, β-Hydroxybutyric acid, type

Compound Identifiers

PubChem CID

441