3-Cyano-7-ethoxycoumarin

SKU: orb1299584

Description

3-Cyano-7-ethoxycoumarin is a fluorogenic substrate selective for cytochrome P450 isoforms CYP1A1 and CYP1A2. It is widely used in high-throughput in vitro assays to measure the enzymatic activity and inhibition of these isoforms in liver microsomes and recombinant systems for drug metabolism research.

Research Area

Metabolism Research

Key Properties

• CAS Number: 117620-77-6
• MW: 215.2
• Purity: >98%
• Formula: C₁₂H₉NO₃
• SMILES: CCOc1ccc2cc(C#N)c(=O)oc2c1
• Target: Cytochrome/MDR
• Solubility: Soluble in DMSO (up to 20 mg/ml with warming) or in Ethanol (up to 3 mg/ml with warming)

Bioactivity

• In Vitro:
  Instructions 1. Solution preparation: 3-Cyano-7-ethoxycoumarin is usually provided in powder form. It can be dissolved in an appropriate solvent (such as DMSO) to prepare a 1-10 mM stock solution. 2. Experimental procedures: 1) Preparation of enzyme reaction system: Add 3-Cyano-7-ethoxycoumarin to the reaction system containing cytochrome P-450 enzyme. The common working concentration is 10-100 µM, but the concentration can be optimized according to the experimental requirements. 2) Appropriate cofactors (such as NADPH) are usually required in the reaction system because cytochrome P-450 requires NADPH as a reducing agent. 3. Enzyme reaction: The reaction system is reacted at an appropriate temperature (usually 37°C) for a period of time, usually 30-60 minutes, and the specific time can be adjusted according to the experimental design. 4. Product detection: After the reaction is completed, the blue fluorescence of the product is detected by a fluorescence spectrophotometer or fluorescence microscope. The excitation wavelength is 410 nm and the emission wavelength is 485 nm, which produces blue fluorescence. 5. Data Analysis: Based on the intensity of the fluorescence signal, the activity of the P-450 enzyme can be analyzed. Higher fluorescence intensity is usually associated with higher enzyme activity.

Storage & Handling

• Storage: RT / protect from light
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, 3Cyano7ethoxycoumarin, 3-Cyano-7-ethoxycoumarin, 3 Cyano 7 ethoxycoumarin

Compound Identifiers

PubChem CID

164045