3α-Aminocholestane

SKU: orb1707752

Description

3α-Aminocholestane (5α-Cholestan-3α-amine) is a potent and selective SHIP1 inhibitor with an IC50 of 2.5 µM. This small molecule is a valuable research tool for studying SHIP1's role in immune cell signaling, applicable in both in vitro biochemical assays and in vivo models of immune regulation and related diseases.

Research Area

Metabolism Research

Key Properties

• CAS Number: 2206-20-4
• MW: 387.68
• Purity: ≥98% (May vary between batches)
• Formula: C₂₇H₄₉N
• SMILES: [H][C@@]1(CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])C[C@H](N)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCCC(C)C
• Target: Phosphatase
• Solubility: DMSO:<0.1 mg/mL;Ethanol:30 mg/mL (77.38 mM)

Bioactivity

• Target IC50:
  SHIP1:2.5 μM
• In Vivo:
  NOD/SCID/γcIL2R (NSG) mutant mice were inoculated with 1 × 10^7 MM cells (OPM2) via intraperitoneal injection. Six hours later, the mice received the first injection of 3α-Aminocholestane (3AC), followed by daily injections for the next 7 days, and subsequently, injections every two weeks for the following 15 weeks. The results indicate that treatment with 3α-Aminocholestane (3AC) inhibits the growth of MM cells in vivo..
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• In Vitro:
  MM cells were treated with increasing concentrations of 3α-Aminocholestane (3AC) for 48 hours. After cell lysis, proteins were separated by SDS-PAGE and probed with antibodies against SHIP1, SHIP2, and PTEN. The results indicate that treatment with3α-Aminocholestane (3AC)leads to proteasomal degradation of the SHIP1 protein.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SHIP1, 5α-Cholestan-3α-amine, 3alpha-Aminocholestane, 3-A-Aminocholestane, 3a-Aminocholestane, 3AC, 3αAminocholestane, 3α-Aminocholestane, 3α Aminocholestane

Compound Identifiers

PubChem CID

5351709