2,5-Di-tert-butylhydroquinone

SKU: orb1299727

Description

2,5-Di-tert-butylhydroquinone (BHQ) is a cell-permeable inhibitor that selectively targets the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA). It is widely used in both in vitro and in vivo studies to investigate calcium signaling, ER stress, and related pathways in cellular physiology and disease models.

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 88-58-4
• MW: 222.32
• Purity: 98.76% (May vary between batches)
• Formula: C₁₄H₂₂O₂
• SMILES: CC(C)(C)C1=CC(O)=C(C=C1O)C(C)(C)C
• Target: Lipoxygenase,COX,Calcium Channel
• Solubility: DMSO:50 mg/mL (224.9 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9 mM)

Bioactivity

• Target IC50:
  Ca2+-ATPase:66.7 μM|COX-2:14.1 μM|5-LOX:1.8 μM
• In Vitro:
  2,5-Di-tert-butylhydroquinone shifted the voltage dependence of the steady-state inactivation curve to more negative potentials by 7 mV in the mid-potential of the curve, without affecting the activation curve as well as the time course of I(Ca(L)) inactivation.Preincubation of the cells either with 10 microM cyclopiazonic acid, a SERCA inhibitor, or with 3 mM diethyldithiocarbamate, an inhibitor of intracellular superoxide dismutase (SOD), did not modify 2,5-Di-tert-butylhydroquinone inhibition of I(Ca(L)).On the contrary, this effect was no longer evident when SOD (250 u ml(-1)) was added to the perfusion medium.Either in the presence or in the absence of cells, 2,5-Di-tert-butylhydroquinone gave rise to superoxide anion formation, which was markedly inhibited by the addition of SOD.At micromolar concentrations, 2,5-Di-tert-butylhydroquinone inhibits vascular I(Ca(L)) by giving rise to the formation of superoxide anion which in turn impairs the channel function.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2,5Ditertbutylhydroquinone, 2,5-Di-tert-butylhydroquinone, 2,5 Di tert butylhydroquinone, Ca2+-ATPase, Calcium Channel, CalciumChannel, BHQ, COX, Cyclooxygenase, inhibit, Lipoxygenase, LOX, Inhibitor

Compound Identifiers

PubChem CID

2374