2',3'-cGAMP sodium

SKU: orb1745284

Description

2',3'-cGAMP sodium is a cyclic dinucleotide second messenger produced by cGAS upon cytosolic DNA sensing. It activates the STING pathway, leading to the induction of type I interferons and pro-inflammatory cytokines. This product is widely used in immunology research to study innate immune signaling in both cellular assays and animal models.

Research Area

Immunology & Inflammation, Metabolism Research

Key Properties

• CAS Number: 2734858-36-5
• MW: 718.37
• Purity: 99.84% (May vary between batches)
• Formula: C₂₀H₂₂N₁₀Na₂O₁₃P₂
• SMILES: OC1([H])[C@](OP(OC[C@](O[C@@H](N2C3=NC=NC(N)=C3N=C2)[C@@H]4O)([H])[C@@]4([H])O5)(O[Na])=O)([H])[C@H](N6C(NC(N)=NC7=O)=C7N=C6)O[C@]1([H])COP5(O[Na])=O
• Target: Endogenous Metabolite,IFNAR,cGAS,STING
• Solubility: DMSO:0.9 mg/mL (1.25 mM);H2O:45 mg/mL (62.64 mM)

Bioactivity

• Target IC50:
  IFN-α (PBMC cells):8.46 μM (EC50)|IFN-β:15 - 42 nM (EC50)|THP-1 cells:> 100 μM|IFN-γ (PBMC cells):7.1 μM (EC50)|TNF-α:36.03 μM (EC50)
• In Vivo:
  At 7 days after tumor cell inoculation, the mice were treated with zebularine alone, 2’,3’-cGAMP sodium alone, or the combination of both. A final concentration of 2.5 mg/mL zebularine was added to drinking water for 10 days until the mice were collected for conducting further experiments. A total of 100 μL of 10μg 2’,3’-cGAMP sodium in PBS at the indicated concentrations was injected into the site next to tumor. 2’,3’-cGAMP sodium treatment was repeated three times with 4-day intervals. This study unveils the role of zebularine in sensitizing the cGAS-STING pathway to promote anti-tumor immunity.
• In Vitro:
  2′3′-cGAMP sodium is capable of enhancing the proinflammatory activation of cultured Wild-type (WT) macrophages. Unlike in macrophages (BMDM), 2′3′-cGAMP sodium treatment displayed anti-inflammatory effects in both WT primary mouse hepatocytes and differentiated 3T3-L1 adipocytes. Specifically, LPS-induced JNK p46 and NF-κB p65 phosphorylation states and IL-1β and TNFα mRNAs in 2′3′-cGAMP sodium -treated WT primary mouse hepatocytes were significantly lower than their respective levels in control-treated hepatocytes. In 3T3-L1 adipocytes, the anti-inflammatory effect of 2′3′-cGAMP sodium was even more pronounced. In particular, LPS-induced JNK p46 phosphorylation states in 2′3′-cGAMP sodium -treated adipocytes were markedly lower than in control-treated adipocytes, and were comparable with JNK p46 phosphorylation states in 2′3′-cGAMP sodium -treated adipocytes in the absence of LPS induction.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Human Endogenous Metabolite, 2',3'-cGAMP sodium, 2'-3'-cyclic GMP-AMP sodium

Compound Identifiers

PubChem CID

145712307