2-NBDG

SKU: orb1308728

Description

2-NBDG

Key Properties

• CAS Number: 186689-07-6
• MW: 342.3
• Purity: >98%
• Formula: C₁₂H₁₄N₄O₈
• SMILES: OC[C@H]1OC(O)[C@H](Nc2ccc([N+]([O-])=O)c3nonc23)[C@@H](O)[C@@H]1O
• Target: Glucose transporter
• Solubility: Soluble in DMSO (up to 10 mg/ml).

Bioactivity

• In Vivo:
  METHODS: Glucose uptake by circulating breast cancer cells was detected by fluorescent microscopy: 1. A mouse blood sample (100 µL/mouse) was collected by puncturing the mouse saphenous vein. 2. Incubate the blood sample containing circulating breast cancer cells with 2-NBDG (5 µg/100 µL blood) for 30 min at 37℃ in a dark incubator. 3. Add the magnetic bead suspension (1µL 1%) to 100 µL of blood sample and incubate for 30 min at 4°C with gentle shaking to promote binding of the magnetic beads to the circulating breast cancer cells. 4. Separate the circulating breast cancer cells from the blood using a magnetic separation rack, wash with PBS 3 times, resuspend in 100 µL PBS and transfer to a 96-well cell plate. 5. 2-NBDG uptake by circulating breast cancer cells was examined under a fluorescence microscope equipped with a 488 nm filter. Large cells with a fluorescent signal derived from fluorescent 2-NBDG uptake by cells were counted as hypermetabolic circulating breast cancer cells, and small-sized normal mouse blood cells (lymphocytes and RBCs) showed no or little 2-NBDG fluorescent signal.
• In Vitro:
  METHODS: Flow cytometry was used to detect glucose uptake: 1, Cells were seeded at 1*104/well in 96-well plates, and the experiment was performed within 24-48 h. The cells were incubated at 37 ℃ for 10-180 min. 2, Remove the cell culture medium, add fresh medium containing 2-NBDG (5-40 µM), and incubate for 10-180 min at 37 ℃. 3. Remove the medium and wash twice with pre-cooled PBS to stop the 2-NBDG uptake reaction. Resuspend in fresh medium and perform flow cytometry within 30 min.
• Cell Research:
  Flow cytometry to detect glucose uptake Operation steps: a. Cells were seeded in 96-well plates at 1*104/well and the experiment was performed within 24-48 h; b. Cell culture medium was removed, fresh culture medium containing 2-NBDG (5-40 µM) was added, and incubated at 37 ℃ for 10-180 min; c. Culture medium was removed, and the cells were washed twice with pre-cooled PBS to stop the 2-NBDG uptake reaction. Resuspended in fresh culture medium, flow cytometry was performed within 30 min.

Storage & Handling

• Storage: -20°C / protect from light
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-NBD-Glucose

Compound Identifiers

PubChem CID

11859095