2-NBDG

SKU: orb1308728

Description

2-NBDG

Key Properties

• CAS Number: 186689-07-6
• MW: 342.26
• Purity: 99.99%
• Formula: C₁₂H₁₄N₄O₈
• SMILES: OC[C@H]1OC(O)[C@H](Nc2ccc([N+]([O-])=O)c3nonc23)[C@@H](O)[C@@H]1O
• Target: Others
• Solubility: H2O:5 mg/mL (14.61 mM);DMSO:52.5 mg/mL (153.39 mM)

Bioactivity

• In Vivo:
  METHODS: Glucose uptake by circulating breast cancer cells was detected by fluorescent microscopy: 1. A mouse blood sample (100 µL/mouse) was collected by puncturin the mouse saphenous vein. 2. Incubat the blood sample containing circulating breast cancer cells with 2-NBDG (5 µg/100 µL blood) for 30 min at 37℃n a dark incubator. 3. Ad the magnetic bead suspension (1µL 1%) to 100 µL of blood sample and incubate for 30 min at 4°C with gentle shaking to promote binding o the magnetic beads to the circulating breast cancer cells. 4. Separat the circulating breast cancer cells fro the blood using a magnetic separation rack, wash with PBS 3 times, resuspend in 100 µL PBS and transfer to a 96-well cell plate. 5. 2-NBDG uptake by circulating breast cancer cells was examined under a fluorescence microscope equipped with a 488 nM filter. Large cells with a fluorescent signal derived from fluorescent 2-NBDG uptake by cells were counted as hypermetabolic circulating breast cancer cells, and small-sized normal mouse blood cells (lymphocytes and RBCs) showed no or little 2-NBDG fluorescent signal.
• In Vitro:
  METHODS: Flow cytometry was used to detect glucose uptake: 1, Cells were seeded at 1*104/well in 96-well plates, an the experiment was performed within 24-48 h the cells were incubated at 37 ℃ for 10-180 min. 2, Remov the cell culture medium, add fresh medium containing 2-NBDG (5-40 µM), and incubate for 10-180 min at 37 ℃. 3. Remov the medium and wash twice with pre-cooled PBS to sto the 2-NBDG uptake reaction. Resuspend in fresh medium and perform flow cytometry within 30 min.
• Cell Research:
  Flow cytometry to detect glucose uptake Operation steps: a. Cells were seeded in 96-well plates at 1*104/well an the experiment was performed within 24-48 h; b. Cell culture medium was removed, fresh culture medium containing 2-NBDG (5-40 µM) was added, and incubated at 37 ℃ for 10-180 min; c. Culture medium was removed, an the cells were washed twice with pre-cooled PBS to sto the 2-NBDG uptake reaction. Resuspended in fresh culture medium, flow cytometry was performed within 30 min.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, 2 NBDG, 2NBDG, 2-NBDG

Compound Identifiers

PubChem CID

11859095