2-Ethynyl-ATP (2-EATP)

• Catalog Number: orb178339
• Category: Tools
• Description: 2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitropolyadenylation of RNA with recombinant poly(A) polymerase. The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click chemistry (CUAAC) that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes); to crosslink the RNA to azide-functionalized biomolecules e.g.proteins.
• Concentration: 10 mM-11 mM
• Form/Appearance: solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Purity: ≥ 90% (HPLC), contains approx. 6% 2-Ethynyl-ADP
• MW: Theoretical MW: 531.20 g/mol; Detected MW: 531.00 g/mol
• SMILES: [C@@H]1([C@@H](O[C@@H]([C@H]1O)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1cnc2c(nc(nc12)C#C)N)O
• Formula: C₁₂H₁₆N₅O₁₃P₃
• Storage: store at -20 °C
• Note: For research use only
• Application notes: in vitro polyadenylation of RNA. ; Spectroscopic Propertie: λmax 265 nm, ε 10.6 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.