2-Deoxy-D-glucose

SKU: orb1300508

Description

2-Deoxy-D-glucose (2-DG) is a glucose analog and glycolytic inhibitor with documented antiviral, anti-proliferative, and pro-apoptotic activities. It is widely used in research applications, including in vitro cell culture and in vivo studies, to investigate cancer metabolism, viral pathogenesis, and cellular stress responses.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 154-17-6
• MW: 164.2
• Purity: >97%
• Formula: C₆H₁₂O₅
• SMILES: OC[C@H]1OC(O)C[C@@H](O)[C@@H]1O
• Target: UPR (Unfolded protein response)
• Solubility: Soluble in Water (up to 50 mg/ml), or in DMSO (up to 15 mg/ml)

Bioactivity

• Target IC50:
  Caenorhabditis elegans growth:19.5 mM|EtHK enzyme:5.66 mM (Km)|HL-60 cells viability:15.55 mM|B-CPAP cells viability:1.154 mM|THP-1 cells viability:16.14 mM|NB4 cells viability:5.75 mM|Kasumi-1 cells viability:0.92 mM
• In Vivo:
  METHODS: To study the effects on leukocyte subpopulation distribution and function, 2-Deoxy-D-glucose (500-1500 mg/kg) was injected intraperitoneally into BDF1 mice once or thrice. RESULTS: Blood glucose concentrations increased in a dose-dependent manner in mice injected with up to 1500 mg/kg of 2-Deoxy-D-glucose. Corticosterone levels, leukocyte counts in the spleen, and CD3+ cells in the thymus increased after one or three injections of 2-Deoxy-D-glucose up to 1500 mg/kg. 2-Deoxy-D-glucose administration induced dose-dependent changes in thymic and splenic cell distribution and function. METHODS: To test the antitumor activity in vivo, 2-Deoxy-D-glucose (1000 mg/kg) was administered intraperitoneally to C57BL/6 mice bearing melanoma B16 once daily for eight days. RESULTS: 2-Deoxy-D-glucose significantly inhibited tumor growth, and TAMs showed a significant decrease in Arg, Fizz, CD206 and Vegf expression after 2-Deoxy-D-glucose treatment.
• In Vitro:
  METHODS: Pancreatic cancer cells MIA PaCa2, BxPC-3, ASPC-1 and ovarian cancer cells OVCAR-3, HEY, SK-OV-3 were treated with 2-Deoxy-D-glucose (0.01-100 mM) for 48 h. Cell proliferation was detected using MTT. RESULTS: 2-Deoxy-D-glucose showed significant antiproliferative activity against tumor cells with IC50 values ranging from 1.45-13.34 mM. METHODS: Triple-negative breast cancer cells Hs578T were treated with 2-Deoxy-D-glucose (15 mM) for 24 days, and cell motility was detected by Migration assay and Invasion assay. RESULTS: 2-Deoxy-D-glucose inhibited the migration and invasion of Hs578T cells.
• Cell Research:
  2×103 H460 or H157 cells are seeded in 96-well cell culture plates. Cells are treated with 5 mM 2-DG only, 5 or 10 μM IGF1R inhibitor II only, or a combination of 2-DG and IGF1R inhibitor II. Cell growth inhibition is determined after 48 h by the CellTiter 96® AQueous nonradioactive cell proliferation assay. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-DG

Compound Identifiers

PubChem CID

439268