1400W dihydrochloride

SKU: orb1304151

Description

1400W dihydrochloride is a potent and selective inhibitor of inducible nitric oxide synthase (iNOS/NOS2). It is widely used in both in vitro and in vivo research to investigate the role of iNOS in inflammation, septic shock, and various cardiovascular and neurological disease models.

Research Area

Cell Biology, Immunology & Inflammation

Key Properties

• CAS Number: 214358-33-5
• MW: 250.17
• Purity: >99.99% (May vary between batches)
• Formula: C₁₀H₁₇Cl₂N₃
• SMILES: Cl.Cl.CC(=N)NCc1cccc(CN)c1
• Target: NO Synthase,Apoptosis,NOS
• Solubility: H2O:139.9 mM;DMSO:255 mg/mL (1019.31 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.99 mM)

Bioactivity

• Target IC50:
  nNOS:2 μM(Ki)|iNOS:<7 nM(Kd)|eNOS:50 μM(Ki)
• In Vivo:
  1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%). 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats. Treatment with 1400W can reduce the rate of growth of solid tumors in mice.
• In Vitro:
  1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS.
• Cell Research:
  RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1400W, 1400W 2HCl, 1400W dihydrochloride, 1400W Dihydrochloride, N-(3-(Aminomethyl)benzyl)acetamidine, NO Synthase, nNOS, Nitric oxide synthases, inhibit, NOS, NOSynthase, eNOS, Inhibitor, iNOS

Compound Identifiers

PubChem CID

2733515