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Catalog Number | orb1261278 |
---|---|
Category | Antibodies |
Description | YAP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 75kDa |
Target | YAP1 |
UniProt ID | P46937 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | YAP1, YAP, YAP2, YAP65, YKI Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using YAP1 antibody (orb1261278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat liver using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using YAP1 antibody (orb1261278) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using PRKCA antibody (orb1261306).
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |