YAP1 Antibody

SKU: orb1261278

Description

YAP1 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
• Target: YAP1
• Molecular Weight: Observed: 75kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

YAP1, YAP, YAP2, YAP65, YKI

Western blot analysis of extracts of various cell lines, using YAP1 antibody (orb1261278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat liver using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody (orb1261278) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using YAP1 antibody (orb1261278) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using PRKCA antibody (orb1261306).

Quick Database Links

Gene Symbol

YAP1

UniProt

P46937