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YAP1 Rabbit Polyclonal Antibody

Catalog Number: orb5629

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5629
CategoryAntibodies
DescriptionYAP1 Rabbit Polyclonal Antibody
TargetYAP1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human YAP1 (131-330/504aa)
UniProt IDP46937
RRIDAB_10918599
MW55 kDa
Tested applicationsICC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative names65 kDa Yes associated protein; YAp 1; YAP 65; YAP;
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NoteFor research use only
YAP1 Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (YAP1) polyclonal Antibody, Unconjugated (orb5629) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

YAP1 Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (YAP1) polyclonal Antibody, Unconjugated (orb5629) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

YAP1 Rabbit Polyclonal Antibody

Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

YAP1 Rabbit Polyclonal Antibody

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

YAP1 Rabbit Polyclonal Antibody

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

YAP1 Rabbit Polyclonal Antibody

Tissue/Cell: human hepatoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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