WNT4 Antibody (Center)

• Catalog Number: orb1929886
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P56705
• MW: 39052 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:2000, IHC-P: 1:10~50, FC: 1:25
• Antibody Type: Primary Antibody
• Clone Number: RB19965
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein Wnt-4, WNT4
• Research Area: Cancer Biology, Signal Transduction, Stem Cell & D
• Note: For research use only
• NCBI: NP_110388.2

Overlay histogram showing MCF-7 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Formalin-fixed and paraffin-embedded human bladder carcinoma reacted with WNT4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-WNT4 Antibody (Center) at 1:2000 dilution. Lane 1: MDA-MB-231 whole cell lysate. Lane 2: mouse bladder lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-WNT4 Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: human testis lysate. Lane 3: mouse bladder lysate. Lane 4: rat lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.