VIM Rabbit Polyclonal Antibody

SKU: orb580337

This product can be custom made

Description

Rabbit polyclonal antibody to VIM

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human VIM
Target	VIM
Protein Sequence	Synthetic peptide located within the following region: LNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRR
Molecular Weight	54kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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FLJ36605

Vimentin Rabbit Polyclonal Antibody [orb158714]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 5
Vimentin Antibody (Center) [orb1931501]
Applications:
FC, IHC-P, WB
Predicted Reactivity:
Hamster, Mouse, Porcine, Rat
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 400 μl
Item 1 of 5
VIM Rabbit Polyclonal Antibody [orb580338]
Applications:
IHC, WB
Predicted Reactivity:
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Sheep, Zebrafish
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl

Quality Guarantee

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Sample Tissue: Mouse Small Intestine, Antibody dilution: 1 ug/ml.

Rabbit Anti-VIM Antibody, Catalog Number: orb580337, Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

Sample Type: Human brain stem cells, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:1000, Color/Signal Descriptions: VIM: Red DAPI:Blue, Gene Name: VIM.

The Vimentin antibody orb580337 works well for visualization of the vimentin cytoskeleton. We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.

WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml, Positive Control: Human brain.

Quick Database Links

Gene Symbol

VIM

UniProt

P08670

RefSeq (Protein):NP_003371

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003371

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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