VHL Antibody

SKU: orb1247872

Description

VHL Antibody

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: EIA, ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant VHL captured by a rabbit antibody. Recommended reporter concentration: 1.5-3µg/mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RSLVKPENYRRLD
• Target: VHL
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome

orb1247872 (1.5 ug/ml) as the reporter antibody.

orb1247872 (0.01 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247872 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

VHL

UniProt

P40337

RefSeq (Protein):NP_000542.1, NP_937799.1